Monoclonal antibody to type F Clostridium botulinum toxin
نویسندگان
چکیده
منابع مشابه
Monoclonal antibody-based immunoassay for type A Clostridium botulinum toxin is comparable to the mouse bioassay.
A monoclonal antibody (BA11) has been produced against Clostridium botulinum type A neurotoxin by the fusion of myeloma cells (P3 NS1/1-Ag4-1) with spleen cells from BALB/c mice immunized with botulinum type A neurotoxoid. The antibody bound specifically to botulinum type A neurotoxin, showing no cross-reactivity with types B and E botulinum toxins or with any of several other bacterial toxins ...
متن کاملPurification and properties of Clostridium botulinum type F toxin.
Clostridium botulinum type F toxin of proteolytic Langeland strain was purified. Toxin in whole cultures was precipitated with (NH4)2SO4. Extract of the precipitate was successively chromatographed on diethylaminoethyl-cellulose at pH 6,0, O-(carboxymethyl) cellulose at pH 4.9, and finally diethylaminoethyl-cellulose at pH 8.1. The procedure recovered 14 percent of the toxin assayed in the star...
متن کاملMolecular construction of Clostridium botulinum type F progenitor toxin.
Molecular dissociation of purified type F progenitor toxin with an S20,W of 10.3 and a molecular weight of 235,000 into two components, toxic and atoxic, was demonstrated by ultracentrifugation, gel filtration, and diethylaminoethyl-Sephadex chromatography at pH 7.5. The ultracentrifugal analysis indicated that type F progenitor toxin dissociates into components of the same molecular size of 5....
متن کاملDevelopment of antibody to each component of Clostridium botulinum type E progenitor toxin.
The passive hemagglutination test with the sheep red blood cells coupled with Clostridium botulinum type E progenitor toxin is highly sensitive and specific to titrate type E botulinum antitoxin. The hemagglutination titers are proportional to the neutralization titers in sera obtained from immunized animals of different species, except for those taken at an early stage after the primary stimul...
متن کاملEpitope characterization of sero-specific monoclonal antibody to Clostridium botulinum neurotoxin type A.
Botulinum neurotoxins (BoNTs) are extremely potent toxins that can contaminate foods and are a public health concern. Anti-BoNT antibodies have been described that are capable of detecting BoNTs; however there still exists a need for accurate and sensitive detection capabilities for BoNTs. Herein, we describe the characterization of a panel of eight monoclonal antibodies (MAbs) generated to the...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1990
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.56.3.808-811.1990